Hamas and Israel are expected to release more hostages and prisoners on the last day of a prolonged six-day truce in the Gaza Strip conflict, as attention focuses on whether mediator Qatar could negotiate another extension. The Palestinian militant group Hamas and allied group Islamic Jihad freed 12 hostages on Tuesday, bringing the total released since the truce began on Friday to 81. Those have been mostly Israeli women and children along with foreign citizens. After they were handed over by the International Committee of the Red Cross (ICRC), the Israeli military said Tuesday's freed hostages, comprising 10 Israeli women and two Thai citizens, received initial medical checks. They then moved to Israeli hospitals where they were to meet their families. The hostages were aged 17 to 84 and included a mother-daughter pair. A short time later, Israel released 30 Palestinians from Ofer Prison in the occupied West Bank and a Jerusalem detention centre. The Palestinian Prisoner's Club, a semi-official organisation, said half were women and the remainder were teenage males. That brought the total number of Palestinians released under the truce to 180. Israel has said the truce could be prolonged further, provided Hamas continues to free at least 10 Israeli hostages per day. But with fewer women and children still in captivity, keeping the guns quiet beyond Wednesday may require negotiating to free at least some Israeli men for the first time. The hostages released on Wednesday were among some 240 people seized by Hamas gunmen during a rampage into southern Israel on October 7 in which Israel says 1200 people were killed. Israel's bombardment of Hamas-ruled Gaza in retaliation has killed more than 15,000 Gazans, health authorities there said. Qatar, which mediated indirect talks between Hamas and Israel that resulted in the ceasefire, on Tuesday hosted the spy chiefs from Israel's Mossad and the United States' CIA. The meeting sought to "build on the progress of the extended humanitarian pause agreement and to initiate further discussions about the next phase of a potential deal", a source briefed on the visits told Reuters. The truce has brought Gaza its first respite after seven weeks of fighting and bombardment that has reduced much of the seaside enclave to rubble. It had been due to expire overnight into Tuesday, but both sides agreed to extend the pause to allow for the release of more Israeli hostages and Palestinian prisoners held by Israel. More than two-thirds of Gaza's 2.3 million people have lost their homes to Israeli bombardments, with thousands of families sleeping rough in makeshift shelters with only the belongings they could carry. They are desperately short of food, fuel and clean water. The temporary ceasefire has allowed about 800 aid trucks to enter Gaza, and the first of three US planes with humanitarian supplies for Gaza landed in Egypt on Tuesday. United Nations aid chief Martin Griffiths was to travel to the Jordanian capital Amman on Wednesday to discuss opening the Kerem Shalom crossing to allow for humanitarian aid to enter Gaza from Israel. Located at the intersection of Israel, the Gaza Strip and Egypt, the Kerem Shalom crossing transported more than 60 per cent of the aid going into Gaza before the current conflict. Aid for Gaza now comes through the Rafah crossing on the Egyptian border, which was designed for pedestrian crossings and not trucks. "We know that more humanitarian aid should be delivered in Gaza. We know how we could increase it, but there are constraints beyond our control," Griffiths told a briefing of member states at the UN in Geneva on Tuesday. "We know that the people of Gaza need much more from us." When the war resumes, Israel has said it intends to press on with its assault from the northern half of Gaza into the south. The White House on Tuesday said Israel faced an "added burden" to protect civilians in the south because many had fled from the north to the south. Australian Associated Press